Part:BBa_K814010

partial scytonemin biosynthetic pathway, scyCB

Our research has focused on novel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB first converts tryptophan into 3-indole pyruvic acid, which is converted into a ketoacid intermediate by ScyA. ScyC then converts the ketoacid into an indole compound which spontaneously forms scytonemin.

This part is an intermediate generated during the making of BBa_K814011. It contains the scyB and scyC generators, but because it lacks scyA, it is not expected to produce scytonemin.

Balskus and Walsh, 2009. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766776/]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2944
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2944
  Illegal NotI site found at 2015
  Illegal NotI site found at 3297
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2944
  Illegal BglII site found at 134
  Illegal BglII site found at 2229
  Illegal XhoI site found at 2023
  Illegal XhoI site found at 3305
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2944
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2944
  Illegal AgeI site found at 794
  
• 1000
  COMPATIBLE WITH RFC[1000]